Dataset
├─Neurons
│ ├─Not stage integrated
│ └─Stage integrated
Stage
Annotations
Display
Size 1
Opacity .3
Gene Expression
0% 100%
0% 100%
0% 100%
Loading gene index…
Differential Gene Expression
👁×
👁×
👁×
Gene set
Sub-UMAP
👁×
Selections
No selections yet. Use the + on a selection box, or save the current selection below.
Connectome matching
No approved annotations loaded.
Main UMAP
Sub UMAP
Select a cluster to view sub-UMAP

Report a Bug

Describe what happened and what you expected. System info is attached automatically.

Paste barcodes

Paste cell barcodes — one per line, or separated by spaces, commas, or tabs. A new selection is created from those found in the current dataset.

…or drop a selection file here — .tsv / .json / .txt
exported selection metadata (rowname column; a “selection” column makes one selection per name), or a plain barcode list. Click to browse.
······

Propose Annotation

Link a cell selection to connectome neurons. Submitted for review; an email copy is sent to the curators.

In Neuroglancer, select your neurons into the Atlas Annotation IDs tab, copy the ID list, and paste it here. (Pasting the whole Neuroglancer URL also works — IDs are read from the Atlas Annotation IDs layer.)
One or more connectome cell types this selection corresponds to. Auto-filled from the pasted neuron IDs above; edit freely.

Review Annotations

VNC Atlas Explorer — Help

Navigation

Zoom in / outScroll
Pan viewDrag
Center & fit viewCenter button
Save UMAP as PNG↓ PNG button

Dataset Selection

Switch dataset (Full VNC, Neurons, Glia)Tree in sidebar
Neuron embeddings: Stage integrated, Not stage integrated, Primary, SecondaryTree sub-items
Filter by developmental stage6h / 24h / 36h / 48h checkboxes

Metadata Colouring

Colour by metadata categoryDropdown selector
Available: Hemilineage, Stage, Sex, Neurogenesis Wave, Soma Neuromere, Fast Transmission, Aminergic Transmission, shTFs Clusters, Fruitless Clones
Hover legend entry to highlight cellsMain & Sub UMAP
Drag legend to repositionDrag header

Cell Selection

Select a group (click point or list item)Click
Add to selection (multi-group)Shift + Click
Lasso select cells on UMAPShift + Drag
Deselect allClick empty area

Gene Expression

Search and overlay gene expression3 colour channels (R/G/B)
Toggle gene visibility / tick button
Adjust expression rangeDual-range slider per gene
Clear gene slot× button
Reload expression dataClick Ready in status bar
Expression source: Full VNC RNA assay for all views

Pre-calculated Sub-UMAPs

Load pre-calculated sub-UMAP for a cluster arrow in list
Colour / shape by metadataDropdowns in sub-UMAP pane
Sex-biased DEGs on hover female / male

Dynamic Sub-UMAP

Open selection box (click to capture cells)Click box in Sub-UMAP section
Accumulate multiple groups into one selectionClick items while box is open
Finalise selectionClick box again or Run
Clear selection× on box
Adjustable parametersParameters tab: Genes, PCs, Neighbors, Min dist, Spread, Epochs
Reproducible: seeded PRNG (seed 42)

Differential Gene Expression

1 Group: selection vs rest of dataset1 Group tab
2 Groups: custom group vs group2 Groups tab
Open box → select cells → close box → RunSame box workflow as Sub-UMAP
Gene set: HVGs (fast) or All genes (comprehensive)Parameters tab toggle
Click colour dot in DEG results to overlay geneR/G/B dots per row
Click same dot again to toggle off
Filter DEG results by gene nameFilter box in results window
Download DEG results or metadataButtons in results window

Neuroglancer 3D Viewer

View hemilineage neurons in MANC connectome3D icon in cluster list
Separate layers for primary / secondary neuronsToggle in NGL viewer
Fru clone 3D view (from Fruitless Clones category)3D button in list
Pop out NGL to new tab button
Close embedded NGL× button

Tooltip

Hover cell for metadata, gene values, fru clone IDsHover
Copy tooltip (includes all neuron IDs for fru clones)Ctrl / + Click

Display

Adjust point sizeSize slider
Adjust point opacityOpacity slider
Toggle dark / light theme / button
Resize main / sub UMAP panesDrag divider

Keyboard Shortcuts

Open this help?
Close this helpEsc